Mass spectrometry imaging of glucosinolates in Arabidopsis flowers and siliques

Mass spectrometry imaging of glucosinolates in Arabidopsis flowers and siliques.

Glucosinolates are multi-functional plant secondary metabolites which play a vital role in plant defence and are, as dietary compounds, important to human health and livestock well-being. Knowledge of the tissue-specific regulation of their biosynthesis and accumulation is essential for plant breeding programs. Here, we report that in Arabidopsis thaliana, glucosinolates are accumulated differe...

Quantification of plant surface metabolites by matrix-assisted laser desorption-ionization mass spectrometry imaging: glucosinolates on Arabidopsis thaliana leaves.

The localization of metabolites on plant surfaces has been problematic because of the limitations of current methodologies. Attempts to localize glucosinolates, the sulfur-rich defense compounds of the order Brassicales, on leaf surfaces have given many contradictory results depending on the method employed. Here we developed a matrix-assisted laser desorption-ionization (MALDI) mass spectromet...

Live Confocal Imaging of Developing Arabidopsis Flowers

The study of plant growth and development has long relied on experimental techniques using dead, fixed tissues and lacking proper cellular resolution. Recent advances in confocal microscopy, combined with the development of numerous fluorophores, have overcome these issues and opened the possibility to study the expression of several genes simultaneously, with a good cellular resolution, in liv...

HPLC Separation of Glucosinolates from Leaves and Seeds of Arabidopsis thaliana and Their Identification Using Thermospray Liquid Chramatography/Mass Spectrometry

Leaf and seed extracts of Arabidopsis thaliana var. Columbia contain a large number of glucosinolates, representing close to 25% of those known to occur in nature. The glucosinolates, in the form of their desulphated analogs, are separated by reversed-phase, high-performance liquid chromatography (HPLC). Seventeen are identified using thermospray liquid chromatography/mass spectrometry (TSP LC/...

Imaging with mass spectrometry.